Next Generation Single-Molecule Biophysics


Using next-generation single-molecule fluorescence techniques, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized  conferences in 2017, 2019, 2022 .


Filius et al, "Full-length single-molecule protein fingerprinting" Nature Nanotechnology (2024)

Severins, Joo, van Noort, "Exploring molecular biology in sequence space: The road to next-generation single-molecule biophysics", Molecular Cell (2022)

Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)

Lab protocols (internal use)

Research practicum (Applied Physics)

Joo lab in Ewha


May 2024 Ivo defends his PhD thesis

Feb 2024 Mike•Raman•Carlos's work published in Nature Nanotechnology (TU Delft, delta, genengnews, phys, newbeezer, myscience)

Feb 2024 Marianne joins our lab for her internship project

Feb 2024 Senne joins our lab for his bachelor project

Feb 2024 Carolien gives a talk in BPS Meeting

Feb 2024 Bhagyashree gives a flash talk in BPS Meeting

Jan 2024 Koushik gives a talk in Single-Molecule Biophysics Les Houches

See also news in 2011- 2023