Next Generation Single-Molecule Biophysics


Using next-generation single-molecule fluorescence techniques, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized conferences in 2017, 2019, 2022 .


Severins, Joo, van Noort, "Exploring molecular biology in sequence space: The road to next-generation single-molecule biophysics", Molecular Cell (2022)

Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)

Filius, Kim, Severins, Joo "High-resolution single-molecule FRET via DNA eXchange" Nano Letters (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)


Feb 2023 Sung Hyun gives a talk in BPS

Feb 2023 Amine starts her master project

Jan 2023 Archana starts her PhD study

Dec 2022 Chirlmin gives Winter School lectures in Ewha University

Oct 2022 We organize a Single-Molecule Protein Sequencing conference in Delft

Oct 2022 Chirlmin gives a talk in International Convention of Pharmaceutical Society

Sep 2022 Chirlmin gives a keynote speech in IMSC 2022

Jul 2022 Chirlmin gives a keynote talk in Gordon Research School

Jun 2022 We are a part of Delft-Erasmus Flagship, Personalized Real-time Health Impact of Climate Change and Pollution.

Jun 2022 Chirlmin receives Award of Excellence from Ministry of Science, Korea

Jun 2022 Kijun awarded EMBO Postdoctoral Fellowship

Jun 2022 Chirlmin affiliated with Ewha University (Distinguished Professor)

May 2022 Chirlmin gives a talk in Ewha University

May 2022 Ivo's review paper published in Molecular Cell

Apr 2022 Chirlmin gives a talk in Peking University

Apr 2022 Our proposal for Doctoral Networks (Marie Skłodowska-Curie Actions) has been accepted

Mar 2022 Raman finishes his master study with cum laude

Mar 2022 Raman receives Gerrit Jan Mulder award

Mar 2022 Moon Hyeok starts his PhD study

Feb 2022 Chirlmin gives a talk in Hong Kong University of Science and Technology

Feb 2022 Chirlmin gives a talk in Korea University

Feb 2022 Ward starts his bachelor thesis project

Jan 2022 Mike defenses his PhD thesis

Jan 2022 Carlos starts his post-doctoral study

See also news in 2011- 2021

Lab protocols (internal use)

Research practicum (Applied Physics)