Next Generation Single-Molecule Biophysics


Using next-generation single-molecule fluorescence techniques, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized  conferences in 2017, 2019, 2022 .


Filius et al, "Full-length single-molecule protein fingerprinting" Nature Nanotechnology (2024)

Severins, Joo, van Noort, "Exploring molecular biology in sequence space: The road to next-generation single-molecule biophysics", Molecular Cell (2022)

Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)

Lab protocols (internal use)

Research practicum (Applied Physics)

Joo lab in Ewha


Oct 2024 Chirlmin gives a talk in Single-Molecule Sensors and NanoSystems International Conference

Oct 2024 Chirlmin gives a talk in HUPO 2024

Jul 2024 Carolien defends her PhD thesis

Jun 2024 Chirlmin gives a talk in EPFL

May 2024 CarolienKijun's paper accepted in Nature Communications

May 2024 Mike gives a talk in Norwegian Symposium on Proteomics

May 2024 Ivo defends his PhD thesis

Mar 2024 Carlos becomes Faculty of Impact fellow (NWO)

Feb 2024 Mike•Raman•Carlos's work published in Nature Nanotechnology (TU Delft, delta, genengnews, phys, newbeezer, myscience)

Feb 2024 Marianne joins our lab for her internship project

Feb 2024 Senne joins our lab for his bachelor project

Feb 2024 Carolien gives a talk in BPS Meeting

Jan 2024 We are awarded ERC Proof of Concept (TU Delft)

Jan 2024 Koushik gives a talk in Single-Molecule Biophysics Les Houches

See also news in 2011- 2023