Single-Molecule Biophysics


Using cutting-edge single-molecule fluorescence tools, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We apply the mechanistic understanding to genome editing.

We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized conferences in 2017 and 2019.


Filius, Kim, Severins, Joo "High-resolution single-molecule FRET via DNA eXchange" Nano Letters (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)

Filius, Cui, Ananth, Docter, Hegge, van der Oost, Joo, "High-speed super-resolution imaging using protein-assisted DNA-PAINT" Nano Letters (2020)

Restrepo, Joo, Dekker "Paving the way to single-molecule protein sequencing" Nature Nanotechnology (2018)


Sep 2021 Koushik starts his PhD study

Sep 2021 Kijun starts his post-doctoral training

Jul 2021 Chirlmin gives a talk in G4 webinar series

May 2021 A new look of the group website!

Apr 2021 Luuk's paper published in Patterns

Apr 2021 Chirlmin gives a webinar in Kent State University

Apr 2021 NWO Vici grant awarded. We are hiring!

Apr 2021 Sungchul starts his group at IBS Center for RNA Research

Apr 2021 Melis starts her bachelor project

Apr 2021 Frank starts his master project

Mar 2021 paper published in eLife

Mar 2021 Hamza•Viktorija's paper published in ACS Chemical Biology

Mar 2021 Mike•Sung Hyun•Ivo's paper published in Nano Letters

Feb 2021 Chirlmin gives a talk in KPS Applied Physics Academy

Feb 2021 Carolien's paper published in RNA Biology

Jan 2021 Chirlmin gives a talk in Dutch Physics Conference

Jan 2021 Jack starts his post-doctoral study

See also news in 2011- 2020

Lab protocols (internal use)

Research practicum (Applied Physics)