Next Generation Single-Molecule Biophysics


Using next-generation single-molecule fluorescence techniques, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized conferences in 2017 and 2019.


Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)

Filius, Kim, Severins, Joo "High-resolution single-molecule FRET via DNA eXchange" Nano Letters (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Filius, Cui, Ananth, Docter, Hegge, van der Oost, Joo, "High-speed super-resolution imaging using protein-assisted DNA-PAINT" Nano Letters (2020)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)


Sep 2022 Chirlmin gives a keynote speech in International Mass Spec Conference

Mar 2022 Moon Hyeok starts his PhD study

Feb 2022 Chirlmin gives a talk in Korea University

Feb 2022 Ward starts his bachelor thesis project

Jan 2022 Mike defenses his PhD thesis

Jan 2022 Carlos starts his post-doctoral study

See also news in 2011- 2021

Lab protocols (internal use)

Research practicum (Applied Physics)