Embedded Files
CHIRLMIN JOO LAB
Using next-generation single-molecule techniques and machine learning, we investigate fundamental biological processes, focusing on the early stages of molecular interactions that are undetectable with conventional biochemistry. Our research spans from small regulatory RNA in gene silencing (RNA interference) and anti-viral defense (CRISPR immunity) to the dynamics of immune recognition, such as MHC-TCR interactions. A key tool in our work is SPARXS (Single-molecule Parallel Analysis for Rapid eXploration of Sequence space), a novel multiplexing single-molecule technique that allows us to probe interactions on a large scale with unprecedented resolution. We also develop single-molecule protein sequencing techniques for advanced proteomics analysis. We have organized conferences in 2017, 2019, and 2022. A new series is in 2025.
KEY PUBLICATIONS
Severins, Bastiaanssen, Kim, et al, C. Joo "Single-molecule structural and kinetic studies across sequence space" Science (2024)
Bastiaanssen, Kim et al "RNA-guided RNA silencing by an Asgard archaeal Argonaute" Nature Communications (2024)
Filius et al, "Full-length single-molecule protein fingerprinting" Nature Nanotechnology (2024)
Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)
Kim, Loeff et al "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)
LAB NEWS
Aug 2025 João starts his PhD project
Aug 2025 KyungLim visits the Delft lab for training
Jul 2025 Sohyun visits the Delft lab for training
Apr 2025 Ewha lab receives NRF funding on epigenetic editors (Next-Gen Bio)
Apr 2025 Dong Hoon•Sung Hyun's manuscript accepted in ACS Nano
Apr 2025 Carolien•Ivo's manuscript accepted in Nature Protocols
Apr 2025 Merel starts her bachelor thesis project
Mar 2025 Sonja starts her bachelor thesis project
Mar 2025 We received funding from ZonMW Open Competition
Mar 2025 Eugene starts his bachelor thesis project
Feb 2025 Mike•Raman's paper published in Science Advances
Feb 2025 We received funding from NWO Open Competition (ENW-M)
Feb 2025 Marianne starts her master thesis project
Feb 2025 Hema starts her bachelor thesis project
Jan 2025 Chirlmin has achieved 10,000 citations with h index 40
Jan 2025 Chirlmin gives a talk in Single-Molecule Protein Sequencing conference
Jan 2025 Chirlmin organizes Single-Molecule Protein Sequencing conference in Bolzano, Italy
Jan 2025 KyungLim visits the Delft lab for training
Jan 2025 Chirlmin gives a talk in KAIST, Korea
See also news in 2011- 2024
Page updated
Report abuse