Single-Molecule Biophysics

CHIRLMIN JOO LAB

Using cutting-edge single-molecule fluorescence tools, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We apply the mechanistic understanding to genome editing.

We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized conferences in 2017 and 2019.

KEY PUBLICATIONS

Alfaro et al, "The emerging landscape of single-molecule protein sequencing technologies" Nature Methods (2021)

Filius, Kim, Severins, Joo "High-resolution single-molecule FRET via DNA eXchange" Nano Letters (2021)

Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)

Filius, Cui, Ananth, Docter, Hegge, van der Oost, Joo, "High-speed super-resolution imaging using protein-assisted DNA-PAINT" Nano Letters (2020)

Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)

LAB NEWS

Sep 2021 Kijun starts his post-doctoral training

Sep 2021 Koushik starts his PhD study

Jul 2021 Chirlmin gives a talk in G4 webinar series

Jun 2021 A review paper on single-molecule protein sequencing published in Nature Methods

Jun 2021 Raman•Mike's paper accepted in Trends in Biochemical Sciences

May 2021 A new look of the group website!

Apr 2021 Luuk's paper published in Patterns

Apr 2021 Chirlmin gives a webinar in Kent State University

Apr 2021 NWO Vici grant awarded. We are hiring!

Apr 2021 Sungchul starts his group at IBS Center for RNA Research

Apr 2021 Melis starts her bachelor project

Apr 2021 Frank starts his master project

Mar 2021 Mike•Carlos's patent published

Mar 2021 FRET.community paper published in eLife

Mar 2021 Hamza•Viktorija's paper published in ACS Chemical Biology

Mar 2021 Mike•Sung Hyun•Ivo's paper published in Nano Letters

Feb 2021 Chirlmin gives a talk in KPS Applied Physics Academy

Feb 2021 Carolien's paper published in RNA Biology

Jan 2021 Chirlmin gives a talk in Dutch Physics Conference

Jan 2021 Jack starts his post-doctoral study