Single-Molecule Biophysics


  • Using cutting-edge single-molecule fluorescence tools, we investigate how small regulatory RNA mediates gene silencing (RNA interference) and anti-viral defense (CRISPR immunity and beyond). We apply the mechanistic understanding to genome editing. 
  • We develop single-molecule protein sequencing techniques for advanced proteomics analysis. We organized  conferences in 2017 and 2019.

  • Kim, Loeff, Colombo, Jergic, Brouns, Joo "Selective loading and processing of prespacers for precise CRISPR adaptation" Nature (2020) (animation)
  • Cui, Klein, Hegge, Chandradoss, van der Oost, Depken, Joo "Argonaute bypasses cellular obstacles without hindrance during target search" Nature Communications (2019)
  • Globyte, Lee, Bae, Kim, Joo "CRISPR/Cas9 searches for a protospacer adjacent motif by lateral diffusion" EMBO Journal (2018)

  • Restrepo, Joo, Dekker "Paving the way to single-molecule protein sequencing" Nature Nanotechnology (2018)

  • van Ginkel, Filius, Szczepaniak, Tulinski, Meyer, Joo "Single-molecule peptide fingerprinting" PNAS (2018)


February 2021
Chirlmin gives a talk in KPS Applied Physics Academy

February 2021 paper accepted in eLife

February 2021
Carolien's paper accepted in RNA Biology

January 2021
Chirlmin gives a talk in Dutch Physics Conference

January 2021
Jack joins as a post-doc

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